Figure 2.

TEER and EC morphology under shear. (a) TEER measurement was performed over 48–52 h post cell seeding in the µSiM-MVM. TEER rises during first 24 h (red curve), after which it remains stable in the absence of shear (blue curve) or progresses to an elevated level in the presence of shear (brown curve). The addition of thrombin immediately causes the TEER to decay below 50% of its maximum value in less than half an hour (black dashed line). Error bars represent standard error of mean with at least n = 5. (b) Endothelial cells grown under static condition for 24 h demonstrate cobblestone morphology as observed in the phase image on the top left, and the corresponding image analysis in MATLAB reveals the isotropic orientation of the cell population. Endothelial cells exposed to shear stress of 10 dynes cm⁻² for 24 h exhibit uniformly aligned morphology with a majority of the cells parallel to the flow direction (Y axis).