Table 1.
Parameters used for the bioreactor cultivations of Schwanniomyces occidentalis and Trichoderma harzianum
| Parameter | S. occidentalis | T. harzianum |
|---|---|---|
| Agitation (rpm) | 300–1200 | 300–1200 |
| Dissolved oxygen control (%) | 20—agitation cascade controlled | 20—agitation cascade controlled |
| Temperature (°C) | 30 | 30 |
| pH | 5.5 ± 0.1 | 4.9 ± 0.1 |
| Acid | 6 M H3PO4—pump speed 20% | 6 M H3PO4—pump speed 20% |
| Base | 6 M NaOH—pump speed 20% | 6 M NaOH—pump speed 20% |
| Feed | YPD | S. occidentalis—pump speed 10% |
| Gasflow (vvm) | 0.5—pressurized air | 0.5—pressurized air |
| Cultivation duration batch phase | 18–22 h—until DO and pH started rising | 36–40 h—until DO and pH started rising |
| Cultivation duration fed-batch phase | 160–180 h total cultivation time | ~ 140–180 h total cultivation time |