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. 2020 Feb 24;13:197–207. doi: 10.2147/CCID.S243154

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© 2020 Nicoll and Buehrer.

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Biguanides, isotretinoin and azithromycin stimulate acute de novo lipogenesis in human primary sebocytes. Sebocytes seeded in Scintiplates were incubated overnight with T0901317 in the presence of increasing concentrations of biguanides, isotretinoin, azithromycin or AICAR. Test articles were reapplied in the presence of insulin with T0901317 and [14C]-acetate for 4 hrs. Sebocytes were washed with PBS and the cell monolayers allowed to dry before [14C]-acetate incorporation was determined by scintillation counting. (A) Dose response of phenformin, buformin and metformin; (B) Dose response of isotretinoin (13-cis RA), azithromycin and AICAR. Controls are uninduced and induced with insulin plus T0901317. Values are plotted as mean ± SD compared to induced control (100%) of three independent determinations; *denotes p < 0.05 compared to induced control.