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. 2020 Feb 24;13:197–207. doi: 10.2147/CCID.S243154

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© 2020 Nicoll and Buehrer.

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Biguanides induce a time dependent increase in de novo neutral lipid synthesis. Sebocytes from three donors in Scintiplates and culture plates were incubated overnight (or 48 hrs) with T0901317 plus phenformin or metformin. Biguanides were reapplied with insulin plus T0901317 and [14C]-acetate for 4 hrs. Scintiplates were analyzed as described previously. Culture plate sebocytes were harvested with trypsin/EDTA and lipid extracts prepared for liquid scintillation counting and TLC analysis of lipid species. (A) [14C]-acetate incorporation induced by phenformin (Phen) using Scintiplate and lipid extraction assays. (B) TLC lipid species analysis: SQ-ChEst, squalene and cholesterol esters; TG, triacylglycerols; DAG/Chol, diacylglycerols and cholesterol; PL, phospholipids. (C) Time course of Scintiplate assay. Values are mean ± SD of three independent cultures. *p<0.05, **p<0.01, ***p<0.001.