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. 2019 Dec;11(6):502–509.

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Copyright© 2019 Iranian Neuroscience Society

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — PCR products obtained using the ISAba-1 forward primer (ISAba-1F) and bla_OXA-like gene reverse primers. (a) Carbapenem-resistant isolates carrying only bla_OXA-51-like after amplification with the ISAba-1F and OXA-51-R primer pair. Lane 1: no chromosomal DNA (negative control). Lanes 2 and 3: DNA of isolates failed to give a band. Lanes 4–7: DNA of isolates with ISAba-1 upstream of bla_OXA-51-like gene with the 1200-bp PCR product. Lane 8: 1-kb DNA ladder. (b) Lane 1: no chromosomal DNA (negative control). Lanes 2–9: DNA of isolates with ISAba-1 upstream bla_OXA-23-like gene with the 1600-bp PCR product. Lane 10: 1-kb DNA ladder. (c) Lane 1: no chromosomal DNA (negative control). Lanes 2–5: DNA of isolates with ISAba-1 upstream of bla_OXA-58-like gene with the 1259-bp PCR product. Lane 6: 1-kb DNA ladder.