Figure 2.

Inflammasome was activated by β-Adrenergic signaling. (A) PKA and pPKA were analyzed by immunoblot from the LV tissue of sham and TAC rats; (B) NRCFs were stimulated with NE for 1, 2, 12, 24 or 48 hours. NLRP3, ASC2 and cleaved caspase-1 were analyzed with immunoblot; (C,D) NRCFs were treated with β-adrenergic receptor blocker (Metoprolol) or PKA inhibitor (H-89) for 30 min prior to NE treatment and analyzed by immunoblots. PKA, protein kinase A; LV, left ventricular; TAC, thoracic aorta constriction; NRCF, neonatal rat cardiac fibroblast; NE, norepinephrine.