Fig. 3.

In vivo tAPC reprogramming significantly inhibits tumor growth and leads to long-term survival. (A) IFN-γ was detectable in the tumor interstitial fluid 14 d after tumor inoculation in treated groups (n = 4). (B) Of mice treated with anti–PD-1, slower tumor growth was measured in groups treated with IL-12 nanoparticles (significance marked by #) or 4-1BBL/IL-12 nanoparticles (significance marked by *). *P < 0.05; ** or ##: P < 0.01; **** or ####: P < 0.0001. Significance was calculated by two-way repeated-measures ANOVA with a Dunnett posttest to compare against animals treated with control nanoparticles and anti–PD-1. (C) Mice treated with IL-12 or 4-1BBL/IL-12 nanoparticles and anti–PD-1 survived significantly longer than the control (P = 0.0018). All error bars are SEM.