Skip to main content
. 2020 Feb 12;117(8):4158–4168. doi: 10.1073/pnas.1917938117

Fig. 2.

Fig. 2.

PTEN−/− spheroids are vulnerable to perturbation of the mevalonate pathway. (A) Cytotoxic effects of pitavastatin on MCF10A and PTEN−/− spheroids in 3D culture. (Scale bar, 30 μm.) (B) MCF10A and PTEN−/− spheroid staining with fluorescein diacetate and propidium iodide after pitavastatin treatment. (Scale bar, 30 μm.) (C) Measurement of the intact spheroids of MCF10A and PTEN−/− in response to increasing concentrations of pitavastatin (mean ± SD, n = 500 spheroids). (D) Measurement of the intact spheroids of PTEN/MCF10A and PTEN/PTEN−/− in response to increasing concentrations of pitavastatin (mean ± SD, n = 500 spheroids). (E) Measurement of the intact spheroids of PTEN−/− in the absence or presence of Mev and increasing concentrations of pitavastatin (mean ± SD, n = 500 spheroids). (F) The addition of GGPP rescues pitavastatin-induced cell death of PTEN−/− in 3D culture. (Scale bars, 30 μm.)