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. 2020 Feb 7;117(8):4180–4187. doi: 10.1073/pnas.1913481117

Fig. 5.

Fig. 5.

Analysis of JMJD2B target genes in EndMT. (A) SULF1 mRNA expression after silencing of JMJD2B in TGF-β2-stimulated ECs using microarray RNA sequencing (n = 3). (B) mRNA levels of SULF1 after siRNA-mediated knockdown of JMJD2B using RT-qPCR, normalized to RPLP0 (n = 4). (C) ChIP-Seq RT-qPCR showing H3K9me3 levels at promoter of SULF1, normalized to IgG (n = 5). (D–F) mRNA levels after siRNA-mediated knockdown of SULF1 or siScr siRNA in HUVECs followed by EndMT. Total RNA was isolated and mRNA levels were determined by RT-qPCR, normalized to GAPDH (2-ΔCt). (D) SULF1, (E) SM22, (F) TGF-β2 expression levels are depicted as fold siScr Ctrl (n = 3). (G) TGF-β2 mRNA level after siRNA-mediated knockdown of JMJD2B in HUVECs. mRNA levels were determined by RT-qPCR, normalized to RPLP0 (2-ΔCt), depicted as fold to siScr Ctrl (n = 9). (H) Cartoon summarizing our findings. Data are depicted as mean ± SEM. Statistical significance was determined using Student’s t test; *P < 0.05.