Fig. 3.

Heat-shock proteases overlap functionally to promote survival during carbon starvation. (A) The heat-shock response is induced during carbon starvation. Gene expression at the indicated time points is relative to 1 h after cells were shifted to CSM and is normalized to the expression of the housekeeping gene oprI. Expression of the DNA replication initiator dnaA was used as a negative control. Error bars show SD of biological replicates (n = 3). (B) Percent survival of protease mutants on day 3 of carbon starvation. The dotted line indicates WT survival. Error bars show SD of biological replicates (n = 3). (C) Survival time course of select mutants during carbon starvation. Representative data from at least two independent experiments are shown.