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Effect of temperature on transfection efficiency
| Temperature | Viability (%) | Fluorescence (%) |
|---|---|---|
| Room temperature | 13.4 ± 0.51 a | 7.3 ± 0.20 a |
| 4 °C | 2.4 ± 0.24 b | 5.8 ± 0.15 b |
Other pulse conditions: 300 V; 1 pulse; pulse duration, 10 ms; 2 mm cuvette; 4 μg DNA; number of replicates = 3. The electroporation buffer was Opti-MEM with GlutaMax
Different superscript letters indicate significant difference (P < 0.05)
