Table 2.
Resistance patterns and mechanisms of resistance
| QRDR Mutations | Resistant genes | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ETT code | STs | CC | Origin | Resistance patern | gyrA | gyrB | grlA | grlB | ERY- DA | GEN | TET | FA |
| 1 | ST954 | CC22 | HUCA | CIP - ERY- DA | S144P | K401R | ermC | |||||
| 2 | ST87 | CC59 | HCP | ERY- TET | msrA | TeT K | ||||||
| 3 | *ST8 | CC5 | HCP | CIP - GEN - TET | S84L, T129I | S144P | K401R | aac(6´)-aph(2") | TeT K | |||
| 4 | ST217 | CC22 | HCP | CIP - ERY | S84L, S85P | S144P, V82S, Y83V, E84R | K401R, D507E | ermC | ||||
| 5 | *ST8 | CC5 | HCP | ERY - TET | msrA | TeT K | ||||||
| 6 | ST45 | CC45 | HCP | CIP - GEN | E134N, L135T | S144P | K401E | aac(6´)-aph(2") | ||||
| 7 | ST22 | CC22 | Hospital del Mar | CIP - ERY - DA | S84L, T129K | Y500T, H501T | S144P, G78A, E84R | ermC | ||||
| 8 | ST22 | CC22 | Hospital del Mar | CIP - ERY | S84L, S85P, T129I | V511A, V303C | S144P, S81P | ermC | ||||
| 9 | ST8 | CC5 | Hospital del Mar | CIP - ERY - GEN - TET | S84L, T129K, I131S | S144P | K401E | msrA | aac(6´)-aph(2") | TeT K | ||
| 10 | ST22 | CC22 | Hospital del Mar | CIP | S84L, S85P | V511A, R447H | S144P | K401R, L440F | ||||
| 11 | ST22 | CC22 | Hospital del Mar | CIP - GEN - TET | S84L, K130G | V511A, G339D, K312T | S144P | K401E, K400Q, D507E | aac(6´)-aph(2") | TeT K | ||
| 12 | ST22 | CC22 | Policlinic Milan | CIP - ERY | S84L, T129K | S144P | K401R | ermC | ||||
| 13 | ST1535 | - | Policlinic Milan | CIP - GEN - TET - FA | R447L | S144P | K403Q, H478Y, D507E | aac(6´)-aph(2") | TeT K | FusB | ||
| 14 | ST22 | CC22 | Policlinic Milan | CIP - ERY | S84L, T129I | H501T | S144P, S80F | ermC | ||||
| 15 | ST83 | CC5 | Policlinic Milan | CIP - ERY | S84L | H501T | S144P | ermC | ||||
| 16 | ST217 | CC22 | Policlinic Milan | CIP - ERY - GEN - DA | S84L | L418F | S144P | K401E | ermA | aac(6´)-aph(2") | ||
| 17 | ST403 | CC5 | Policlinic Milan | CIP | T129K | V511A, S425G, R447N | S144P | D503E, A504P | ||||
| 18 | ST1221 | CC5 | Policlinic Milan | CIP - ERY | S84L, E88A | S144P, S80F | K401E, D507N | ermC | ||||
| 19 | ST22 | CC22 | Policlinic Milan | CIP - ERY - GEN - DA | S84L | S144P | K403Q, A504P, D507E | ermA | aac(6´)-aph(2") | |||
* ETT collected at different ICU. ETT code 3, 5, and 6 = Methicillin sensitive Staphylococcus aureus
Abbreviations: CC Clonal Complex, CIP Ciprofloxacin, DA Clindamycin, ERY Erythromycin, FA Fusidic acid, GEN Gentamicin, HCP Hospital Clinic (Spain), HUCA Hospital Universitario Central de Asturias (Spain); Policlinic Milan, Fondazione IRCCS Ca′ Granda (Italy), QRDR Quinolone Resistance-Determining Region, ST sequence type; Strain 20, susceptible to all antimicrobial agents tested (ST217, CC22), TET Tetracycline