Table 3.
Autoantigens identified in HTM cells, captured by autoantibodies of CTRL sera (Group ID: ‘CH’)
| No. | Entry name (*_HUMAN) | UniProt accession | Protein name | Sequence coverage (%) | No. of peptides | No. of unique peptides | iBAQ (%) |
|---|---|---|---|---|---|---|---|
| 1 | FLNA | P21333 | Filamin‐A | 11 | 20 | 16 | 1.24 |
| 2 | TBB6 | Q9BUF5 | Tubulin beta‐6 chain | 46.6 | 15 | 6 | 6.51 |
| 3 | SYEP | P07814 | Bifunctional glutamate/proline‐tRNA ligase | 11 | 12 | 12 | 1.15 |
| 4 | SET | Q01105 | Protein SET | 19.3 | 3 | 3 | 8.23 |
| 5 | TBB4B | P68371 | Tubulin beta‐4B chain | 51.7 | 17 | 1 | 5.88 |
| 6 | SYIC | P41252 | Isoleucine‐tRNA ligase, cytoplasmic | 12.1 | 12 | 12 | 0.99 |
| 7 | FAS | P49327 | Fatty acid synthase | 4.8 | 7 | 7 | 0.44 |
| 8 | TERA | P55072 | Transitional endoplasmic reticulum ATPase | 12.8 | 6 | 6 | 1.14 |
| 9 | H4 | P62805 | Histone H4 | 35.9 | 4 | 4 | 14.39 |
| 10 | XRCC5 | P13010 | X‐ray repair cross‐complementing protein 5 | 23.8 | 8 | 8 | 2.28 |
| 11 | RS2 | P15880 | 40S ribosomal protein S2 | 18.8 | 4 | 4 | 3.09 |
| 12 | H2B1L | Q99880 | Histone H2B type 1‐L | 19 | 2 | 2 | 9.69 |
| 13 | HNRPF | P52597 | Heterogeneous nuclear ribonucleoprotein F | 11.3 | 3 | 2 | 4.65 |
| 14 | RS16 | P62249 | 40S ribosomal protein S16 | 14.4 | 2 | 2 | 4.6 |
| 15 | SYTC | P26639 | Threonine‐tRNA ligase, cytoplasmic | 11.1 | 7 | 7 | 1.77 |
| 16 | PLPHP | O94903 | Pyridoxal phosphate homeostasis protein | 17.5 | 3 | 3 | 1.81 |
| 17 | HS90B | P08238 | Heat shock protein HSP 90‐beta | 20.9 | 10 | 7 | 1.41 |
| 18 | ATPA | P25705 | ATP synthase subunit alpha, mitochondrial | 15.9 | 5 | 5 | 1.56 |
| 19 | SERPH | P50454 | Serpin H1 | 14.1 | 4 | 4 | 1.6 |
| 20 | NPM | P06748 | Nucleophosmin | 12.6 | 3 | 3 | 3.29 |
| 21 | MCM7 | P33993 | DNA replication licensing factor MCM7 | 7.4 | 4 | 4 | 0.55 |
| 22 | CCD47 | Q96A33 | Coiled‐coil domain‐containing protein 47 | 14.3 | 4 | 4 | 2.03 |
| 23 | ADT2 | P05141 | ADP/ATP translocase 2 | 14.4 | 4 | 2 | 1.69 |
| 24 | SYRC | P54136 | Arginine‐tRNA ligase, cytoplasmic | 17.7 | 10 | 10 | 0.74 |
| 25 | IF4A1 | P60842 | Eukaryotic initiation factor 4A‐I | 27.8 | 6 | 6 | 1.69 |
| 26 | IQGA1 | P46940 | Ras GTPase‐activating‐like protein IQGAP1 | 2.8 | 2 | 2 | 0.1 |
| 27 | HNRPK | P61978 | Heterogeneous nuclear ribonucleoprotein K | 7.8 | 3 | 3 | 1.01 |
| 28 | EIF3L | Q9Y262 | Eukaryotic translation initiation factor 3 subunit L | 3 | 1 | 1 | 0.54 |
| 29 | SYK | Q15046 | Lysine‐tRNA ligase | 8.9 | 4 | 4 | 0.5 |
| 30 | SYDC | P14868 | Aspartate‐tRNA ligase, cytoplasmic | 7.8 | 4 | 4 | 0.66 |
| 31 | SRP68 | Q9UHB9 | Signal recognition particle subunit SRP68 | 12.1 | 5 | 5 | 0.51 |
| 32 | TBA1A | Q71U36 | Tubulin alpha‐1A chain | 46.1 | 17 | 1 | 1.07 |
| 33 | RS24 | P62847 | 40S ribosomal protein S24 | 9 | 1 | 1 | 2.63 |
| 34 | RL15 | P61313 | 60S ribosomal protein L15 | 5.9 | 1 | 1 | 1.3 |
| 35 | EIF3E | P60228 | Eukaryotic translation initiation factor 3 subunit E | 2.9 | 1 | 1 | 0.45 |
| 36 | EIF3F | O00303 | Eukaryotic translation initiation factor 3 subunit F | 5.3 | 1 | 1 | 0.76 |
| 37 | SYQ | P47897 | Glutamine‐tRNA ligase | 9 | 5 | 5 | 0.36 |
| 38 | TCPZ | P40227 | T‐complex protein 1 subunit zeta | 7.2 | 2 | 2 | 0.47 |
| 39 | MYO1C | O00159 | Unconventional myosin‐Ic | 1.2 | 1 | 1 | 0.05 |
| 40 | EIF3A | Q14152 | Eukaryotic translation initiation factor 3 subunit A | 3.5 | 4 | 4 | 0.16 |
| 41 | DEST | P60981 | Destrin | 10.3 | 1 | 1 | 1.63 |
| 42 | EIF3B | P55884 | Eukaryotic translation initiation factor 3 subunit B | 3.4 | 1 | 1 | 0.26 |
| 43 | SYMC | P56192 | Methionine‐tRNA ligase, cytoplasmic | 1.2 | 1 | 1 | 0.33 |
| 44 | NSUN2 | Q08J23 | tRNA (cytosine(34)‐C(5))‐methyltransferase | 3.9 | 2 | 2 | 0.43 |
| 45 | COPG1 | Q9Y678 | Coatomer subunit gamma‐1 | 4.9 | 2 | 2 | 0.2 |
| 46 | MCM3 | P25205 | DNA replication licensing factor MCM3 | 2.7 | 2 | 2 | 0.13 |
| 47 | PSMD2 | Q13200 | 26S proteasome non‐ATPase regulatory subunit 2 | 4.5 | 2 | 2 | 0.12 |
| 48 | OLA1 | Q9NTK5 | Obg‐like ATPase 1 | 3.8 | 1 | 1 | 0.19 |
| 49 | RS5 | P46782 | 40S ribosomal protein S5 | 13.7 | 1 | 1 | 0.9 |
| 50 | ACTN1 | P12814 | Alpha‐actinin‐1 | 3.8 | 3 | 3 | 0.27 |
| 51 | PYRG1 | P17812 | CTP synthase 1 | 2.7 | 1 | 1 | 0.19 |
| 52 | PPIL4 | Q8WUA2 | Peptidyl‐prolyl cis‐trans isomerase‐like 4 | 8.7 | 2 | 2 | 0.34 |
| 53 | NU160 | Q12769 | Nuclear pore complex protein Nup160 | 1.3 | 1 | 1 | 0.06 |
| 54 | P5CS | P54886 | Delta‐1‐pyrroline‐5‐carboxylate synthase | 4.5 | 2 | 2 | 0.14 |
| 55 | TKT | P29401 | Transketolase | 6.9 | 2 | 2 | 0.21 |
| 56 | DCTN1 | Q14203 | Dynactin subunit 1 | 3.7 | 3 | 3 | 0.08 |
| 57 | CLH1 | Q00610 | Clathrin heavy chain 1 | 0.5 | 1 | 1 | 0.07 |
| 58 | PRP8 | Q6P2Q9 | Pre‐mRNA‐processing‐splicing factor 8 | 0.9 | 1 | 1 | 0.03 |
| 59 | DX39A | O00148 | ATP‐dependent RNA helicase DDX39A | 4.9 | 2 | 2 | 0.31 |
| 60 | TIM50 | Q3ZCQ8 | Mitochondrial import inner membrane translocase subunit TIM50 | 2.8 | 1 | 1 | 0.4 |
| 61 | CPSF7 | Q8N684 | Cleavage and polyadenylation specificity factor subunit 7 | 5.3 | 1 | 1 | 0.25 |
| 62 | SPT5H | O00267 | Transcription elongation factor SPT5 | 1.7 | 1 | 1 | 0.07 |
| 63 | IMB1 | Q14974 | Importin subunit beta‐1 | 1 | 1 | 1 | 0.06 |
| 64 | RT35 | P82673 | 28S ribosomal protein S35, mitochondrial | 3.1 | 1 | 1 | 0.17 |
| 65 | TGFI1 | O43294 | Transforming growth factor beta‐1‐induced transcript 1 protein | 4.3 | 1 | 1 | 0.11 |
| 66 | DDX46 | Q7L014 | Probable ATP‐dependent RNA helicase DDX46 | 1 | 1 | 1 | 0.05 |
Proteins were reproducibly identified in all three replicates. MaxQuant iBAQ values were used to calculate protein abundance.