Figure 3.
Effects of Ufm1 on NF-κB p65 expression in RAW264.7 cells. Immunoblotting results showing cytoplasmic (A) and nuclear (B) NF-κB p65 expression in Lv-shUfm1-, Lv-shNC-, Lv-Ufm1- and Lv-NC-treated RAW264.7 cells. LPS: 1000 ng/mL; TNF-α: 20 ng/mL. The levels of cytoplasmic (A1-A2) and nuclear (B1-B2) p65 were quantified and are shown as the fold change compared to the control (0 min). The data are presented as the mean±SD, n=3. ***P<0.001 compared to the Lv-shNC group; ##P<0.01 compared to the Lv-NC group.