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. 2020 Feb 25;14:795–810. doi: 10.2147/DDDT.S238695

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© 2020 Hu et al.

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Ufm1 promotes TNF/LPS-dependent NF-κB activation by promoting the ubiquitination and degradation of IκBα. (A) Effects of Ufm1 on p-IKK and p-IκBα levels in RAW264.7 cells; (B) Effects of Ufm1 on the ubiquitination of IκBα. The cells were incubated with 50 µg/mL ALLN for 30 min and then treated with LPS (1000 ng/mL) and TNF-α (20 ng/mL). Cytoplasmic extracts were fractionated and then subjected to Western blot analysis using specific anti-IκBα antibodies. (C) Effects of Ufm1 on the degradation of IκBα. IκBα levels were quantified and are shown as the fold change compared to the control at 0 min. The data are presented as the mean±SD, n=3. **P<0.01 compared to the Lv-shNC group; ^##P<0.01 compared to the Lv-NC group.