Table 2.
Inter-Day and Intra-Day (Precision and Accuracy) of the Developed Methodology
| HLM Matrix | 5 ng/mL (LLQC) | 15 ng/mL (LQC) | 150 ng/mL (MQC) | 400 ng/mL (HQC) | ||||
|---|---|---|---|---|---|---|---|---|
| Inter-Day Assaya | Intra-Day Assayb | Inter-Day Assay | Intra-Day Assay | Inter-Day Assay | Intra-Day Assay | Inter-Day Assay | Intra-Day Assay | |
| Mean | 4.99 | 4.98 | 14.85 | 14.75 | 151.6 | 152.20 | 400.95 | 402.21 |
| SD | 0.23 | 0.15 | 0.37 | 0.27 | 1.90 | 2.02 | 6.12 | 2.71 |
| Precision (% RSD) | 4.70 | 3.04 | 2.49 | 1.82 | 1.26 | 1.33 | 1.53 | 0.67 |
| % Accuracy | −0.18 | −0.38 | −0.98 | −1.66 | 1.07 | 1.47 | 0.24 | 0.55 |
Notes: aAverage of six repeats for 3 days. bAverage of 12 repeats on the same day.