FIG. 3.

Data sampling in-sequence is required to recapitulate experimental agent motile behavior. (a) The spatial trajectory of a representative highly persistent NanoBEADS agent experimental agent, (b) the spatial trajectory of an agent simulated by random sampling, (c) persistence vs time for a single agent (red curve) by random sampling from a single tracked experimental agent (black curve), and (d) the spatial trajectory of the agent by sampling in sequence from the data for the same experimental agent. Note that once all experimental data have been sampled (without replacement), all data are reset to permit simulations of indefinite duration.