TABLE I.
Model parameters.
Parameter | Variable | Value | Source |
---|---|---|---|
Average run duration in the absence of a chemoeffector gradient | 0.86 s | 63 | |
Average tumble duration | 0.14 s | 63 | |
Chemotactic sensitivity for l-aspartic acid | 35 s | Estimated | |
Dissociation constant for l-aspartic acid | 18 μM | Estimated | |
Bacteria swimming speed | 34 μm/s | Measured | |
Mean change in bacteria bearing between successive run phases | 68° | 63 | |
Standard deviation of bacteria bearing change between run phases | 36° | 63 | |
Basal QS signal (AHL) generation rate | 3.19 molecules/s | Fitted | |
Upregulated QS signal (AHL) generation rate | 234 molecules /s | Fitted | |
Hill constant | 2.5 | 65 | |
QS signal (AHL) upregulation threshold | 1.87 nM | Fitted | |
Rate of GFP translation | 4.0 × 10−1 molecules/s | Fitted | |
Rate of GFP maturation | 3.02 × 10−3 s−1 | Estimated69 | |
Maximum rate of GFP degradation | 5.54 molecules/s | Estimated70,71 | |
Half-maximum concentration for GFP degradation | 6650 molecules/cell | Estimated70 | |
Diffusion coefficient of the QS signaling molecule (AHL) in water | 490 μm2/s | 72 | |
Rate of QS signal (AHL) degradation | 10.8% h−1 | 73 |