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. 2019 Aug 14;105(3):610–622. doi: 10.3324/haematol.2019.218503

Figure 4.

Figure 4

Effect of PIEZO1 activation on the transcriptional program of erythroid differentiation in primary human CD34+-derived cells, assessed by quantitative reverse transcriptase polymerase chain reaction. For all experiments, primary cells were cultured for 10 days, with 1 μM YODA1 or dimethylsulfoxide (DMSO) stimulation from day 3 to 10. Gene expression was assessed relative to GAPDH expression. (A) Compared to exposure to DMSO, exposure to 1 μM YODA1 decreased GPA mRNA expression (×0.49±0.17), β-globin RNA expression (×0.4±0.26) and α-globin RNA expression (×0.3±0.24). (B) Compared to exposure to DMSO, exposure to 1 μM YODA1 increased the GATA2/GATA1 mRNA ratio (×6.2±1.9). (C) Stimulation with 1 μM YODA1 increased STAT5A and BMI-1 expression, and decreased EPOR, SLC4A1, ALAS2, and AHSP mRNA expression. (n=3 for all experiments); ***P<0.001; **P<0.01; *P<0.05.