Skip to main content
. 2019 Jun 27;105(3):598–609. doi: 10.3324/haematol.2019.221804

Figure 4.

Figure 4

ELANE knockout restored granulocytic differentiation of ELANE-CN iPSC. (A) Flow cytometry analysis of suspension cells harvested from embryoid body (EB)-based granulocytic cell culture of respective iPSC clones on day 28 or 32 of differentiation. Data represent means ± standard deviation (SD) from two independent experiments. *P<0.05, **P<0.01. (B) Wright-Giemsa staining of cytospin preparations of suspension myeloid cells harvested from iPSC culture at day 28 or 32 of differentiation. Representative images are depicted. (C) Colony-forming unit (CFU) assay of CD34+ cells harvested from EB-based iPSC culture on day 14 of differentiation. Data represent means ± SD from two independent experiments. *P<0.05.