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. 2019 Jun 27;105(3):598–609. doi: 10.3324/haematol.2019.221804

Figure 8.

Figure 8

Human ELANE KO PMN are capable to migrate and to phagocyte S. aureus BioParticles in zebrafish embryos in vivo. (A) The scheme of in vivo phagocytosis assay in zebrafish embryos xenotransplanted with human fluorescently labeled polymorphonuclear leukocytes (PMN). (B) A representative confocal image highlighting the presence of transplanted human ELANE KO PMN in the caudal hematopoietic site of a zebrafish embryo at 53 hpf. White arrows indicate S. aureus BioParticles phagocytosed by human ELANE KO PMN. CV: caudal vein; DA: dorsal aorta. Scale bar: 20 mm. (C) Three-dimensional rendering of a z-stacks of 12 mm illustrating human ELANE KO PMN, one of them has engulfed S. aureus BioParticles (white arrow). Scale bar: 10 μm. (D) Still photographs from a time-lapse recording illustrating the phagocytic activity of transplanted human ELANE KO PMN in the zebrafish embryo. Arrowheads indicate the formation of neutrophil protrusions. Numbers indicate time in minutes. Scale bar: 10 μm.