Table 4.

Arithmetic mean tick counts and percent efficacy relative to placebo following one oral treatment with Simparica Trio^™ for dogs with existing Ixodes scapularis infestations and after subsequent weekly re-infestations

Study	Tick strain	Daya	Placebob		Simparica Trio™b		% Efficacy	Test statistic
			Mean	Range	Mean	Range
7	Oklahoma State Universityc	2	21.9	14–34	0	0–0	100	t(17) = 12.10, P < 0.0001
		7	13.8	3–20	0	0–0	100	t(17) = 9.34, P < 0.0001
		14	19.6	5–31	0	0–0	100	t(18) = 8.72, P < 0.0001
		21	20.6	13–34	1.0	0–10	95.1	t(9) = 8.63, P < 0.0001
		28	21.6	9–37	0	0–0	100	t(17) = 6.64, P < 0.0001
		35	23.2	10–35	0	0–0	100	t(18) = 9.85, P < 0.0001
8	Wild-caught ticksd	2	26.7	20–36	0	0–0	100	t(9) = 16.02, P < 0.0001
		7	27.2	22–35	0	0–0	100	t(9) = 19.47, P < 0.0001
		14	25.5	19–32	0	0–0	100	t(9) = 20.27, P < 0.0001
		21	26.6	22–34	0.1	0–1	99.6	t(9) = 21.84, P < 0.0001
		28	24.8	19–33	0	0–0	100	t(9) = 17.18, P < 0.0001
		35	26.8	19–37	0	0–0	100	t(9) = 17.73, P < 0.0001

^aFirst infestation on Day -2. Subsequent infestations on Day 5, 12, 19, 26 and 33. Tick counts were conducted at 48 hours post-treatment and weekly re-infestation

^bTreatment with placebo or Simparica Trio^™ (minimum dose 1.2 mg/kg sarolaner, 24 µg/kg moxidectin and 5 mg/kg pyrantel (as pamoate salt)) occurred on Day 0

^cColony originated from ticks field-collected in Stillwater, OK, USA, in 1991 and was last supplemented with wild-caught ticks 10 months prior to study start

^dWild-caught adult ticks collected in South Carolina, USA, during the two months prior to study start