Table 5.

Arithmetic mean tick counts and percent efficacy relative to placebo for dogs following one oral treatment with Simparica Trio^™ with existing Rhipicephalus sanguineus infestations and after subsequent weekly re-infestations

Study	Tick strain	Daya	Placebob		Simparica Trio™b		% Efficacy	Test statistic
			Mean	Range	Mean	Range
9	Bertek Incc	2	23.8	12–40	0.1	0–1	99.6	t(9.02) = 7.59, P < 0.0001
		7	17.3	9–28	1.0	0–10	94.2	t(9) = 8.24, P < 0.0001
		14	11.7	3–17	1.2	0–12	89.7	t(18) = 5.98, P < 0.0001
		21	15.1	4–26	0	0–0	100	t(18) = 7.00, P < 0.0001
		28	14.6	4–26	0	0–0	100	t(17) = 6.46, P < 0.0001
		35	12.8	4–26	0	0–0	100	t(17) = 5.50, P < 0.0001
10	Ecto Services Incd	2	34.7	29–46	0.1	0–1	99.7	t(9) = 22.01, P < 0.0001
		7	29.4	19–43	0.1	0–1	99.7	t(9.03) = 12.80, P < 0.0001
		14	21.1	15–30	0.1	0–1	99.5	t(9) = 13.95, P < 0.0001
		21	23.1	13–39	0.7	0–3	97.0	t(9.46) = 8.89, P < 0.0001
		28	24.6	20–37	0.2	0–1	99.2	t(9) = 16.78, P < 0.0001
		35	24.9	19–33	1.5	0–6	94.0	t(12.3) = 13.68, P < 0.0001

^aFirst infestation on Day -2. Subsequent infestations on Day 5, 12, 19, 26 and 33. Tick counts were conducted at 48 hours post-treatment and weekly re-infestation

^bTreatment with placebo or Simparica Trio^™ (minimum 1.2 mg/kg sarolaner, 24 µg/kg moxidectin and 5 mg/kg pyrantel (as pamoate salt)) occurred on Day 0

^cColony originated from ticks field-collected in Greenbrier, AR, USA, in 2006 and was last supplemented with wild-caught ticks ~ 2 years prior to study start

^dColony originated from ticks collected in 1991 from dogs in Stillwater, OK, USA, and was obtained from Professional Laboratory and Research Service, Inc (PLRS), Corapeake, NC, USA. Last supplementation of the colony with wild-caught ticks was 2–3 months prior to study start