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. 2020 Feb 18;2020:1528362. doi: 10.1155/2020/1528362

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Copyright © 2020 Kun Liu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Qualitative and quantitative western blot and mRNA expressive levels of DNMT1, DNMT3a, and DNMT3b after treatment with S, S + HBO, CCI, and CCI + HBO groups.(a) DNMT3a protein showed time-dependent increased expression with the highest point on the 14th day in L4 and L5 DRG, but not DNMT1 and DNMT3b (n = 6 rats per group for three repeats, ^∗presented compared with the S group, P < 0.05). (b) Western blot and qt-PCR also presented that DNMT3a became gradually stronger after CCI. And the protein and mRNA expression of DNMT3a in the CCI group was stronger than that in the CCI + HBO group (n = 6 rats per group for three repeats, ^∗presented compared with the S group, P < 0.05). (c) Western blot in the CCI group is stronger than that in the CCI + HBO group (^∗presented compared with the S group, P < 0.05). Qualitative and quantitative protein and mRNA expression of DNMT1 and DNMT3b after treatment in all groups had no significant difference (n = 6 rats per group for three repeats, P < 0.05).