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. 2020 Feb 17;44(1):15–23. doi: 10.3906/biy-1909-12

Figure 1.

Figure 1

Doxorubicin regulates PD-L1 mRNA and protein in HCT116 and MDA-MB-231 cells in an opposite way. HCT116 and MDA- MB-231 cells were incubated with various concentrations of doxorubicin (DOX) (for HCT116 cells (+) 0.1 ?M, (++) 0.5 ?M, (+++) 1.5 ?M; for MDA-MB-231 cells (+) 0.7 ?M) or without any treatment for 18 h. A) The mRNA expression of PD-L1 in DOX-treated or nontreated HCT116 and MDA-MB-231 cells was assessed by qRT-PCR. Data are normalized to 18S rRNA and are representative of 3 independent experiments. P-values were calculated via unpaired t-test (**P < 0.01; ***P < 0.001). B) Protein expression of PD-L1 in DOX-treated or nontreated HCT116 and MDA-MB-231 cells evaluated by immunoblotting. Vinculin was used as a loading control. Immunoblots shown are representative of 3 independent experiments. n.s. denotes a nonspecific band. C) Densitometric quantification of PD-L1 protein expression levels in DOX-treated and nontreated HCT116 and MDA-MB-231 cells was performed by normalizing to vinculin.