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. 2020 Feb 17;44(1):15–23. doi: 10.3906/biy-1909-12

Figure 3.

Figure 3

Doxorubicin regulates miR-140 expression in HCT116 and MDA-MB-231 cells in an opposite way. HCT116 and MDA- MB-231 cells were kept with various concentrations of DOX (for HCT116 cells (++) 0.5 ?M, (+++) 1.5 ?M; for MDA-MB-231 cells (+) 0.7 ?M) or without any treatment for 18 h. A) The expression of miR-34a in DOX-treated or nontreated HCT116 and MDA-MB-231 cells assessed by qRT-PCR. B) Schematic image of PD-L1 and miRNA-140 interaction. C) The expression of miR-140 in DOX-treated or nontreated HCT116 and MDA-MB-231 cells assessed by qRT-PCR. Data are normalized to U6 and are representative of 3 independent experiments. P values were calculated by unpaired t-test (**P < 0.01; ***P < 0.001. D) The correlations of PD-L1 with miR-140 were assessed. All values are expressed as means SEM. Data are normalized to U6. The data are representative of 3 independent experiments. P values are calculated by unpaired t-test (*P < 0.05, **P < 0.01; ***P < 0.001, ****P < 0.0001).