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. 2020 Feb 17;44(1):15–23. doi: 10.3906/biy-1909-12

Figure 4.

Figure 4

miR-140 binds and partially regulates PD-L1 in HCT116 cells. HCT116 cells were kept untreated (dotted histogram) or transfected either with p2FP-RNAi vector that included the 3’ UTR region of PD-L1 (p2FP-PD-L1 3’ UTR shaded histogram) or p2FPRNAi vector-only control (p2FP-RNAi-solid histogram). The p2FP-RNAi vector expresses two fluorescent proteins: TurboGFP and JRed. A) Representative histogram of JRed and GFP expression in untreated, p2FP-RNAi, and p2FP-PD-L1 3’ UTR. B) Net expression of JRed and GFP in groups shown in (A). C) HCT116 cells were kept untreated (-) or transfected with both p2FP-PD-L1 3’ UTR vector and with either control miRNA mimic (Neg) or miRNA-140 mimic (miRNA-140). Representative contour plot that depicts the percentage of GFP-positive cells. D) GFP/JRed mean ratio is shown for groups shown in (C). All values are expressed as means ± SEM. Significance of the data was calculated using one-way analysis of variance (ANOVA). P values are as follows: *P < 0.05; **P < 0.01.