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. 2020 Jan 10;11(3):549–560. doi: 10.1111/1759-7714.13280

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© 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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MiR‐22‐3p reverses the promotion of NNT‐AS1 on NSCLC progression. (a–h) H1299 and A549 cells were transfected with pcDNA, NNT‐AS1, NNT‐AS1+miR‐NC or NNT‐AS1+miR‐22‐3p. (a and b) The level of miR‐22‐3p was detected by qRT‐PCR. (c and d) Cell proliferation was evaluated by MTT assay. (e–h) The migrated and invaded cells were detected using transwell assay. (i and j) The protein levels of E‐cadherin, N‐cadherin and Vimentin were examined by western blot assay. *P < 0.05. pcDNA, NNT‐AS1, NNT‐AS1+miR‐NC, NNT‐AS1+miR‐22‐3p.

Inline graphic — MiR‐22‐3p reverses the promotion of NNT‐AS1 on NSCLC progression. (a–h) H1299 and A549 cells were transfected with pcDNA, NNT‐AS1, NNT‐AS1+miR‐NC or NNT‐AS1+miR‐22‐3p. (a and b) The level of miR‐22‐3p was detected by qRT‐PCR. (c and d) Cell proliferation was evaluated by MTT assay. (e–h) The migrated and invaded cells were detected using transwell assay. (i and j) The protein levels of E‐cadherin, N‐cadherin and Vimentin were examined by western blot assay. *P < 0.05. pcDNA, NNT‐AS1, NNT‐AS1+miR‐NC, NNT‐AS1+miR‐22‐3p.