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. 2020 Jan 10;11(3):549–560. doi: 10.1111/1759-7714.13280

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© 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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YAP1 knockdown abrogates the promotion effects of NNT‐AS1 overexpression on NSCLC progression. (a and b) The protein level of YAP1 was detected in H1299 and A549 cells transfected with miR‐NC, miR‐22‐3p, miR‐22‐3p+pcDNA or miR‐22‐3p+NNT‐AS1. (c–j) H1299 and A549 cells were transfected with pcDNA, NNT‐AS1, NNT‐AS1+sh‐NC or NNT‐AS1+sh‐YAP1. (c and d) Cell viability was detected by MTT assay. (e–h) The migrated and invaded cells were measured by transwell assay. (i and j) The protein levels of p‐ERK and EMT‐related proteins were detected by western blot assay. *P < 0.05. pcDNA, NNT‐AS1, NNT‐AS1+sh‐NC, NNT‐AS1+sh‐YAP1.

Inline graphic — YAP1 knockdown abrogates the promotion effects of NNT‐AS1 overexpression on NSCLC progression. (a and b) The protein level of YAP1 was detected in H1299 and A549 cells transfected with miR‐NC, miR‐22‐3p, miR‐22‐3p+pcDNA or miR‐22‐3p+NNT‐AS1. (c–j) H1299 and A549 cells were transfected with pcDNA, NNT‐AS1, NNT‐AS1+sh‐NC or NNT‐AS1+sh‐YAP1. (c and d) Cell viability was detected by MTT assay. (e–h) The migrated and invaded cells were measured by transwell assay. (i and j) The protein levels of p‐ERK and EMT‐related proteins were detected by western blot assay. *P < 0.05. pcDNA, NNT‐AS1, NNT‐AS1+sh‐NC, NNT‐AS1+sh‐YAP1.