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. 2020 Jan 9;11(3):537–548. doi: 10.1111/1759-7714.13274

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© 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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CircCDR1as is a decoy of miR‐219a‐5p in NSCLC cells. (a) The binding sites of circCDR1as and miR‐219a‐5p were predicted by starBase. (b and c) Luciferase activity was measured in A549 and Calu‐3 cells transfected with circCDR1as‐WT or circCDR1as‐MUT and miR‐NC or miR‐219a‐5p. () miR‐NC and () miR‐219a‐5p. (d and e) The expression of miR‐219a‐5p was detected in A549 and Calu‐3 cells transfected with pcDNA, circCDR1as, si‐NC or si‐circCDR1as#1 by qRT‐PCR. (d) () pcDNA and () circCDR1as. (e) () si‐NC and () si‐circCDR1as#1. (f) The expression of miR‐219a‐5p was measured in NSCLC tissues and normal tissues (n = 30) by qRT‐PCR. (g) The level of miR‐219a‐5p was detected in A549 and Calu‐3 cells by qRT‐PCR. (h) The linear relationship between the levels of miR‐219a‐5p and circCDR1as in NSCLC tissues was analyzed. *P < 0.05.

Inline graphic — CircCDR1as is a decoy of miR‐219a‐5p in NSCLC cells. (a) The binding sites of circCDR1as and miR‐219a‐5p were predicted by starBase. (b and c) Luciferase activity was measured in A549 and Calu‐3 cells transfected with circCDR1as‐WT or circCDR1as‐MUT and miR‐NC or miR‐219a‐5p. () miR‐NC and () miR‐219a‐5p. (d and e) The expression of miR‐219a‐5p was detected in A549 and Calu‐3 cells transfected with pcDNA, circCDR1as, si‐NC or si‐circCDR1as#1 by qRT‐PCR. (d) () pcDNA and () circCDR1as. (e) () si‐NC and () si‐circCDR1as#1. (f) The expression of miR‐219a‐5p was measured in NSCLC tissues and normal tissues (n = 30) by qRT‐PCR. (g) The level of miR‐219a‐5p was detected in A549 and Calu‐3 cells by qRT‐PCR. (h) The linear relationship between the levels of miR‐219a‐5p and circCDR1as in NSCLC tissues was analyzed. *P < 0.05.