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. 2020 Jan 9;11(3):537–548. doi: 10.1111/1759-7714.13274

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© 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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SOX5 is a target of miR‐219a‐5p in NSCLC cells. (a) The binding sites of miR‐219a‐5p and SOX5 were predicted by TargetScan. (b and c) Luciferase activity was detected in A549 and Calu‐3 cells transfected with SOX5‐WT or SOX5‐MUT and miR‐NC or miR‐219a‐5p. () miR‐NC and () miR‐219a‐5p. (d–g) The mRNA and protein levels of SOX5 were measured in A549 and Calu‐3 cells transfected with miR‐NC, miR‐219a‐5p, anti‐miR‐NC or anti‐miR‐219a‐5p by qRT‐PCR and western blot. (d, e) () miR‐NC and () miR‐219a‐5p. (f, g) () anti‐miR‐NC and () anti‐miR‐219a‐5p. (h and i) The expression of SOX5 at mRNA and protein levels was examined in NSCLC tissues and normal tissues (n = 30). (j and k) The mRNA and protein levels of SOX5 were measured in A549 and Calu‐3 cells by qRT‐PCR and western blot. (l) The linear correlation between levels of miR‐219a‐5p and SOX5 in NSCLC tissues was analyzed. *P < 0.05.

Inline graphic — SOX5 is a target of miR‐219a‐5p in NSCLC cells. (a) The binding sites of miR‐219a‐5p and SOX5 were predicted by TargetScan. (b and c) Luciferase activity was detected in A549 and Calu‐3 cells transfected with SOX5‐WT or SOX5‐MUT and miR‐NC or miR‐219a‐5p. () miR‐NC and () miR‐219a‐5p. (d–g) The mRNA and protein levels of SOX5 were measured in A549 and Calu‐3 cells transfected with miR‐NC, miR‐219a‐5p, anti‐miR‐NC or anti‐miR‐219a‐5p by qRT‐PCR and western blot. (d, e) () miR‐NC and () miR‐219a‐5p. (f, g) () anti‐miR‐NC and () anti‐miR‐219a‐5p. (h and i) The expression of SOX5 at mRNA and protein levels was examined in NSCLC tissues and normal tissues (n = 30). (j and k) The mRNA and protein levels of SOX5 were measured in A549 and Calu‐3 cells by qRT‐PCR and western blot. (l) The linear correlation between levels of miR‐219a‐5p and SOX5 in NSCLC tissues was analyzed. *P < 0.05.