Figure 2.
Circ_0007255 existed in cytoplasm with stable characteristics. (a) Back‐splice junction was detected in cDNA synthesized using random primers but not Oligo dT(18) primed RNA. MB231 (
) random primers and (
) oligo (dT)18 primers. MB468 (
) random primers and (
) oligo (dT)18 primers. (b) QRT‐PCR assay was performed to analyze the levels of circ_0007255, GAPDH, and U6 in the cytoplasm and nucleus in MB231 and MB468 cells. MB231 (
) cytoplasm and (
) nuclear. MB468 (
) cytoplasm and (
) nuclear. (c and d) The levels of circ_0025202 and GAPDH expression in BC cells were reckoned after treating with RNase R or Actinomycin D. Data are presented as mean ± SD of three independent tests. *P < 0.05. (c) MB231 (
) RNase R− and (
) RNase R+. MB468 (
) RNase R− and (
) RNase R+. (d) MB231 (
) circ_0007255 and (
) mKIF4A. MB468 (
) circ_0007255 and (
) mKIF4A.