Figure 6.
SIX2 was a direct target of miR‐335‐5p. (a) The complementary sequences between miR‐335‐5p and SIX2 were predicted by StarBase. (b) Dual‐luciferase reporter assay was conducted to test the luciferase activity of WT‐SIX2 and MUT‐SIX2 reporters in MB231 and MB468 cells with miR‐335‐5p or miR‐NC transfection. MB231 (
) miR‐NC and (
) miR‐335‐5p. MB468 (
) miR‐NC and (
) miR‐335‐5p. (c–f) The mRNA and protein expression of SIX2 in BC tissues and cells was determined. (g) Knockdown efficiency of anti‐miR‐335‐5p was identified using qRT‐PCR assay. (
) Anti‐miR‐NC and (
) anti‐miR‐335‐5p. (h and i) The effect of miR‐335‐5p or anti‐miR‐335‐5p transfection on the level of SIX was analyzed in BC cells. All data are presented as mean ± SD, and each test was repeated three times. *P < 0.05. (h) (
) miR‐NC, (
) miR‐335‐5p, (
) anti‐miR‐NC and (
) anti‐miR‐335‐5p. (i) (
) miR‐NC, (
) miR‐335‐5p, (
) anti‐miR‐NC and (
) anti‐miR‐335‐5p.