Figure 7.
MiR‐335‐5p regulated the progression of BC by targeting SIX2. Si‐NC, si‐SIX2, si‐SIX2+miR‐NC, or si‐SIX2+miR‐335‐5p was transfected into MB231 and MB468 cells, respectively. (a and b) Relative level of SIX2 in MB231 and MB468 cells transfected with SIX2 and miR‐335‐5p is presented. (c) Oxygen consumption ratio was detected by Seahorse extracellular flux analysis in BC cells. MB231 (
) si‐NC, (
) si‐SIX2, (
) si‐SIX2+anti‐miR‐NC and (
) si‐SIX2+anti‐miR‐335‐5p. MB468 (
) si‐NC, (
) si‐SIX2, (
) si‐SIX2+anti‐miR‐NC and (
) si‐SIX2+anti‐miR‐335‐5p. (d) The capacity of colony formation was exhibited in MB231 and MB468 cells. (e and f) The effect of SIX2 and circ_0007245 on cell migration and invasion was assayed in BC cells. Data are presented as mean ± SD of three independent assays. *P < 0.05.