Figure 4.

Inhibition of Nrf‐2 promotes the sensitivity of EC109 cells to cisplatin. (a) Eca109 cells transfected with control or Nrf‐2 siRNA were treated with different concentrations of cisplatin (0–32 μg/mL) for 24 hours, and the cell viability was measured by CCK8 assay. IC₅₀ values of cisplatin in cells are shown (b). Eca109 cells transfected with control or Nrf‐2 siRNA were treated with 2 μg/mL cisplatin for 24 hours, and cell apoptosis was detected by FCM with double staining with by Annexin and PI (c, d). Mean (±standard deviation) values from three independent experiments are shown (*P < 0.05 vs. control; #P < 0.05, control cells treated with cisplatin compared to Nrf‐2 siRNA cells treated with cisplatin). () NC siRNA, () NRF‐2 siRNA.