Table 1.
Main E. coli BL21(DE3) pET37b(+)/pspA4Pro cultivation conditions for batch bioreactor Experiments #1 to #8, cultivation time (tf) to reach the highest PspA4Pro production (PP), and the corresponding biomass concentration (CX). Data for Experiments #2, #5, #7 (I and II), and #8 are available as Supplementary Material.
| Exp # | Medium/T/Inducer | tf | CX | PP | Reference |
|---|---|---|---|---|---|
| h | gDCW/L | gPspA4Pro/L | |||
| 1 | DM/27 °C/IPTG | 25.5 | 32.8 ± 1.8 | 3.1 ± 0.5 | [38] |
| 2 | DM/27 °C/LAC | 27.5 | 34.5 ± 0.6 | 2.6 ± 0.3 | This work |
| 3 | DM/32 °C/IPTG | 16.3 | 31.3 ± 1.6 | 3.8 ± 0.4 | [38] |
| 4 | DM/37 °C/IPTG | 14.6 | 27.0 ± 1.0 | 3.4 ± 0.3 | [38] |
| 5 | DM/37 °C/LAC | 19.5 | 20.4 ± 0.3 | 1.3 ± 0.0 | This work |
| 6 | CM-ANS/31 °C/LAC | 16.4 | 38.0 ± 0.7 | 5.4 ± 0.3 | This work |
| 7.I | CM-VNS/31 °C/LAC | 16.6 | 38.3 ± 0.7 | 5.6 ± 0.3 | [37] |
| 7.II | CM-VNS/31 °C/LACa | 16.0 | 39.2 ± 0.7 | 6.0 ± 0.3 | This work |
| 8 | CM-VNS/31 °C/LACb | 15.0 | 31.8 ± 2.3 | 4.7 ± 0.7 | This work |
CM-ANS: Complex medium with animal nitrogen source; DCW: Dry cell weight; DM: Defined medium; Exp #: Experiment number; LAC: lactose; PspA4Pro: Pneumococcal surface Protein A from clade 4; T: Culture temperature; CM-VNS: Complex medium with vegetal nitrogen source. a: Replication of experiment #7.I; b: Alternative vegetal nitrogen source.