Table 2.
Concentrations and costs of each medium component used in the bioreactor cultures.
| Component | Purchase Cost (US$/kg) |
Concentrations for each experiment |
|||
|---|---|---|---|---|---|
| Units | 1, 3, and 4 | 2 and 5 | 6, 7, and 8 | ||
| Glucosea | 0.7 | g/L | 10.0 | ||
| Glycerola | 1.0 | g/L | 60.0 | 60.0 | 60.0 |
| Yeast extracta | 1.7 | g/L | 5.0 | ||
| MgSO4.7H2Oa | 0.3 | g/L | 0.4 | 0.4 | 0.5 |
| Na2HPO4a | 2.0 | g/L | 9.0 | ||
| KH2PO4b | 1.2 | g/L | 17.7 | 17.7 | 3.4 |
| NH4Cla | 0.2 | g/L | 2.7 | ||
| (NH4)2HPO4a | 0.4 | g/L | 5.3 | 5.3 | |
| Citric acida | 1.2 | g/L | 2.3 | 2.3 | |
| Na2SO4a | 0.1 | g/L | 0.7 | ||
| Kanamycinb | 1.0 | mg/L | 100.0 | 100.0 | 100.0 |
| Ferric citrateb | 6.0 | mg/L | 133.3 | 133.3 | 100.8 |
| CoCl2.6H2Ob | 12.7 | mg/L | 3.3 | 3.3 | 2.5 |
| MnCl2.4H2Ob | 1.7 | mg/L | 20.0 | 20.0 | 15.0 |
| CuCl2.2H2Oa | 4.2 | mg/L | 2.0 | 2.0 | 1.5 |
| H3BO3a | 0.6 | mg/L | 4.0 | 4.0 | 3.0 |
| NaMoO4.2H2Oa | 9.9 | mg/L | 2.8 | 2.8 | 2.1 |
| Zn(CH3CHOOH).H2Ob | 1.7 | mg/L | 33.8 | 33.8 | 33.8 |
| EDTAa | 1.9 | mg/L | 18.8 | 18.8 | 14.1 |
| Thiaminea | 36.1 | mg/L | 45.0 | 45.0 | 45.0 |
| Polypropylene glycola | 3.6 | g/L | 0.3 | 0.3 | 0.3 |
| Peptonea | 6.1 | g/L | 10.0 | ||
| Soy supplementc | 12.2 | g/L | 10.0 | ||
| Lactosea | 2.0 | g/L | 20.0 | 20.0 | |
| IPTGb | 601 | mmol/L | 1.0 | ||
| Total medium expenses | US$/L | 0.24 | 0.15 | 0.21d/0.27e | |
Sources of raw material costs: a: COSTDRIVERS; b: Molbase; c: Soy supplement supplier. d: Peptone (Experiments #6 and #7); e: Soy Supplement (Experiment #8).