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. 2019 Jul 7;10(3):475–487. doi: 10.1016/j.apsb.2019.07.001

Figure 3.

Figure 3

Effects of ProBiotic-4 treatment on intestinal tight junction proteins and inflammation in aged SAMP8 mice. (A) Representative photomicrographs of HE staining and immunostaining of intestine tight junction markers claudin-1, occludin, and ZO-1 in the intestine. (B)–(D) Quantitative image analysis of claudin-1, occludin, and ZO-1 protein expressions based on the integrated optical density (IOD). (E) PCR analysis of Il-6 and Tnf-α mRNA levels in the intestine. The relative mRNA levels of Il-6 and Tnf-α were normalized to Gapdh. The results are expressed as the normalized fold change relative to vehicle-treated SAMR1 group. (F) and (G) IL-6, TNF-α and LPS levels in Plasma were measured by ELISA. The data are expressed as mean ± SEM (n = 6–7/group). *P < 0.05, **P < 0.01 versus SAMP8 group treated with vehicle (one-way ANOVA followed by the Tukey post hoc test).