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. 2019 Jul 7;10(3):475–487. doi: 10.1016/j.apsb.2019.07.001

Figure 5.

Figure 5

ProBiotic-4 reduced oxidative DNA damage and inhibited RIG-I activation in the brain in aged SAMP8 mice. (A) Representative immunoblots and quantitative analysis of γ-H2AX expression in the brain tissues. The levels of γ-H2AX was normalized to β-actin. The results are expressed as the normalized optical density value relative to the vehicle-treated SAMR1 group. (B) The DNA oxidation product 8-OHdG level in the brain was measured by ELISA. (C) Representative immunoblots and quantitative analysis of RIG-I expression in the brain tissues. The levels of RIG-I was normalized to β-actin. The results are expressed as the normalized optical density value relative to vehicle-treated SAMR1 group. (D) Representative immunoblots of RIG-I multimerization in the brain tissues. The data are expressed as mean ± SEM (n = 5–6/group). *P < 0.05, **P < 0.01 versus SAMP8 group treated with vehicle (one-way ANOVA followed by the Tukey post hoc test).