Figure 3.

Reversal of BA inhibition of osteoclast differentiation by IL-1β treatment. (A) BMMs were cultured in the presence of M-CSF and were incubated with RANKL for 2 days in the presence of 10 μmol/L BA. The amount of IL-1β in the culture media was determined by ELISA. (B) and (C) BMMs were cultured in the presence of M-CSF and were exposed to RANKL in the presence of 10 μmol/L BA and the indicated concentrations of IL-1β (B) or PGE2 (C). The cells were fixed, subjected to TRAP staining and observed under a light microscope. Scale bar, 200 μm. TRAP-positive MNCs were counted. ^**P < 0.01 and ^***P < 0.001 control vs. IL-1β (B) or control vs PGE2 (C) in the absence and presence of BA, or between the indicated groups.