Figure 5.

BA inhibition of cell migration and bone resorption, and their rescue by IL-1β treatment. (A) BMMs were cultured in the presence of M-CSF and were further incubated with RANKL for 15 h after gentle scratching in the absence and presence of 10 μmol/L BA or IL-1β (20 ng/mL). The cells migrating to the scratched area were counted. (B–D) BMMs were cultured in the presence of M-CSF and were treated with RANKL for 12 days on dentin discs (B) or 2 days (C and D). The cells were removed from the dentin discs and the resorption pits were visualized by staining with hematoxylin (B). The cell lysates were subjected to immunoblotting analysis (C). The individual gene transcription was quantified by real-time PCR and presented as fold induction (D). Scale bar, 200 μm *P < 0.05 and ^***P < 0.001 between the indicated groups. ns, not significant.