Figure 7.

Effects of BA on osteoblast differentiation and mineralization. Calvaria cells were cultured in OGM for 12 days (A-upper panel and B), 21 days (A-lower panel and C) or the indicated times (D). (A) The cells were fixed in 4% paraformaldehyde and stained for ALP (upper panel) or with ARS (lower panel). (B) Cell lysates were subjected to ALP activity assay. (C) The ARS bound to cells was dissolved and the released ARS was measured. (D) The individual gene transcription was quantified by real-time PCR and presented as fold induction. *P < 0.05, ^**P < 0.01 and ^***P < 0.001 between the indicated groups. ns, not significant.