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. 2019 Nov 8;10(3):462–474. doi: 10.1016/j.apsb.2019.11.004

Figure 8.

Figure 8

Inhibition of LPS- or OVX-induced bone destruction by BA. (A–C) LPS (12.5 mg/kg body weight) alone or with BA (10 mg/kg), each in a 100-μL volume of vehicle (PBS), was injected into the space between the subcutaneous tissue and the periosteum in the skulls of mice at days 0 and 2. Five days after first injection, the calvariae of mice were fixed, stained with TRAP and decalcified. (A) Images of TRAP and hematoxylin-stained calvaria. Scale bar, 2 mm. (B) The calvaria was embedded in paraffin, sectioned and stained with TRAP and hematoxylin. TRAP-positive cells are marked by arrow. Scale bar: 100 ×, 100 μm; 400 ×, 50 μm. (C) Bone cavity (left panel) and TRAP-positive osteoclasts (right panel) were quantified and expressed as fold difference. (D–G) Mice underwent either sham operation or OVX, and BA (10 mg/kg) was administrated to a group of mice intraperitoneally six times a week for three weeks. Sections of tibia were stained with TRAP and hematoxylin. Scale bar: 40 × , 100 μm; 100 × , 50 μm (D). The regions of TRAP-positive cells below the growth plate were measured. OcS/BS, osteoclast surface to bone surface (E). Representative micro-CT images of femurs: upper, sagittal; middle, three-dimensional reconstruction; bottom, transaxial; Scale bar, 0.5 mm (F). Histomorphometric analysis of femurs: BMD, bone mineral density; BS/TV, bone surface density; BS/BV, bone surface to bone volume; Tb.N, trabecular number; Tb.Th, trabecular thickness; BV/TV, bone volume density; Tb.Pf, trabecular pattern factor; Tb.Sp, trabecular spacing (G). n = 5. *P < 0.05, **P < 0.01 and ***P < 0.001 between the indicated groups. ns, not significant.