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. 2019 Sep 5;10(3):447–461. doi: 10.1016/j.apsb.2019.08.006

Figure 2.

Figure 2

Berberine modulated the function of EGCs and decreased the expression of substance P in vitro. (A)–(C), Rat EGC cell line, CRL-2690, were treated with indicated concentrations of berberine. (A) Western blot assay of GFAP and GDNF of EGCs at various timepoints. (B) The mRNA expression level of Gfap and Gdnf of EGCs after 4 h incubation was determined, *P < 0.05, **P < 0.01, and ***P < 0.001 compared with medium control. (C) Immunofluorescence staining with GFAP and GDNF in EGC (Scale = 25 μm). (D) and (E) Rat splenocytes were activated with ConA stimulation for 24 h, and subsequently the supernatants were added into EGC cells culture. (D) Apoptosis assay of EGCs following annexin V and PI staining after 24 h incubation. (E) The mRNA expression of substance P and Gdnf of EGCs. (F) CRL-2690 cells were culture with recombinant BDNF (100 μg/mL) in the presence or absence of indicated concentrations and real-time PCR was performed to determine the expression of substance P. (E) and (F) *P < 0.05, **P < 0.01, and ***P < 0.001 compared with splenocytes supernatants or BDNF-primed cells. All data were presented as mean ± SEM of three independent experiments.