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. 2019 Sep 5;10(3):447–461. doi: 10.1016/j.apsb.2019.08.006

Figure 4.

Figure 4

Berberine suppressed the colonic leukocytes infiltration by regulating chemotaxis and adhesion. The percentage of macrophage (CD11b+F4/80+), neutrophils (CD11b+Gr-1+), and dendritic cells (CD11b+CD11c+) were assayed in MLNs (A) and colonic lamina propria (B). (C) Colonic sections were immunofluorescence stained with Ly6G, CD11b, and F4/80 and the nuclei were stained with DAPI (Scale = 100 μm). (D) The percentage of CD25-positive cells, naïve T cells (CD44‒CD62L+) and effector T cells (CD44+CD62L‒) gated on CD3+CD4+ subpopulation, Th1 (CD4+IFN-γ+) and Th17 cells (CD4+IL-17+) in MLNs. (E) The percentage of CD3+CD4+ cells, CD3+γδTCR+ cells, and CD3+IL-17+ cells in colonic lamina propria. (F) Heatmap of the mRNA expression of chemokines, chemokine receptors, and adhesion molecules. (G) Immunofluorescence staining with CCR6 and DAPI of colonic tissue (Scale = 100 μm). (H) Western blot assay of certain chemokines, chemokine receptors, and adhesion molecules expression. All data were presented as mean ± SEM; n = 8 mice per group.