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. 2019 Oct 18;10(3):488–497. doi: 10.1016/j.apsb.2019.10.004

Figure 1.

Figure 1

CHMFL-VEGFR2-002 inhibits VEGFR2 activity. (A) IC50 determination of CHMFL-VEGFR2-002 with purified VEGFR2 kinase protein using the ADP-Glo assay. (B) GI50 determination of CHMFL-VEGFR2-002 with TEL-VEGFR2-BaF3 cells using the CCK8 assay. (C) EC50 determination of CHMFL-VEGFR2-002 on auto-phosphorylation of VEGFR2 in TEL-VEGFR2-BaF3 cells. (D) The cellular on-target effect of CHMFL-VEGFR2-002 using the Cellular Thermal Shift Assay. (E) Docking of CHMFL-VEGFR2-002 into VEGFR2 kinase X-ray crystal structure (PDB ID: 4AG8).