Skip to main content
. 2019 Oct 18;10(3):488–497. doi: 10.1016/j.apsb.2019.10.004

Figure 3.

Figure 3

Anti-angiogenesis effect of CHMFL-VEGFR2-002 in vitro. (A) CHMFL-VEGFR2-002 inhibited phosphorylation of VEGFR2 in HUVEC cells. (B) CHMFL-VEGFR2-002 impaired capillary tube formation of HUVEC cells. HUVECs were plated on Matrigel with DMSO or various concentrations of compounds. After 24 h, tubes were photographed and measured. (C) CHMFL-VEGFR2-002 inhibited HUVEC invasion in transwell assay. 5 × 104 HUVECs were seeded in the top chamber with various concentrations of compounds. After 24 h, the invaded HUVEC cells were photographed and counted. (D) CHMFL-VEGFR2-002 inhibited HUVEC cell migration in wound healing assay. CHMFL-VEGFR2-002 group is compared with DMSO group, and significance of difference is indicated. The distance of migration is measured by ImageJ. Data are mean±SD (n=3). *P < 0.05, **P < 0.01, ***P < 0.001 versus control treatment.