Figure 4.

Transcriptome Feature of Spermatogonia Cells following Tet1 Deficiency or Aging

(A) PCA analysis of TPM showing differential gene expression pattern among 3 month-old WT and Tet1^−/−, and 11 month-old WT and Tet1^−/− spermatogonia and few spermatocytes.

(B) Correlation analysis. X axis represented the log10(TPM+1) of 11 month-old WT group, and Y axis represented the 1og10(TPM+1) of 3 month-old Tet1^−/− spermatogonia cells. Their correlation coefficient was 0.99.

(C) Differential gene expression profile shown by pheatmap.

(D) GO enrichment analysis of specific genes with high expression levels in 3 month-old WT mice.

(E) Pheatmap showing the genes enriched in spermatogenesis and germ cell development.

(F) GO enrichment analysis of specific genes with high expression levels in 11 month-old Tet1^−/− males.

(G) Pheatmap illustrating the genes enriched in nucleic acid binding transcription factor activity and immune system process.

See also Figures S5 and S6.