Figure 2.

Myrcludex B binds squirrel monkey NTCP. HEK293 cells were transfected with protein expression vectors that express species‐specific NTCPs. (A) Two days after transfection, the cells were either lysed for western blot analysis of NTCP expression. (B) NTCP‐expressing HEK293 cells were incubated with fluorescently labeled Myrcludex B (left column). Fluorescent Myrcludex B binding could be blocked by pre‐incubation with unlabeled Myrcludex B (right column). Abbreviations: DAPI, 4´,6‐diamidino‐2‐phenylindole (a type of stain).