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. 2020 Jan 20;48(5):2502–2517. doi: 10.1093/nar/gkz1237

Figure 4.

Figure 4.

LASTR depletion leads to widespread intron retention. (A) Histogram showing the distributions of differences in percent of intron retention (PIR) and exon skipping (PSI) events in MDA-MB-231 expressing shGFP or shSART3-2. (B) Histogram showing the distributions of differences in percent of intron retention (PIR) and exon skipping (PSI) events in MCF10A cells transfected with Scramble GapmeR or LASTR GapmeRs 1 and 2. (C) RT-qPCR analysis of LASTR expression in MDA-MB-231 cells infected with tetracycline-inducible shRNAs against LASTR, untreated or treated with doxycycline (1 μg/ml, 8 days). Data are presented as mean ± s.e.m.; P-value was determined by a two-sided t-test, n = 3. (D–F) Histograms showing the distribution of differences in percent of intron retention (PIR) and exon skipping (PSI) in MDA-MB-231 cells infected with tetracycline-inducible shRNAs against LASTR, untreated or treated with doxycycline (1 μg/ml, 8 days). (G) Histogram showing the distribution of differences in percent of the indicated splicing events in hypoxic (1% O2, 48 h) MCF10A cells transfected with Scramble GapmeR or LASTR GapmeRs 1 and 2. (A, B, D–G) The number of identified intron retaining and exon skipping events is shown. P-value was determined by Binomial test.